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As a natural resinous substance collected by honeybees from buds and exudates of trees, propolis is used by bees as a glue, general-purpose sealer, and draught extruder for beehives. In this paper, different extraction methods were employed to compare their efficiency in the extraction of bee propolis samples. The methods employed using ethanol as a solvent were the following: soaking method, ultrasonication method, and microwave method. Gas chromatography-mass spectrometry (GC-MS) and spectroscopic methods such as absorbance and fluorescence were utilized to determine the amount of phenolic compounds extracted and compare each extraction's efficiency method. Results showed samples obtained from ultrasonication and microwave methods gave the highest yields. Both methods can be performed within a short time in comparison to the soaking method.more » « less
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